Tg3(krt4:GFP) and Tg(tp63:Tol2-Tp63-AcGFP) at 3 days post-fertilization were dissociated into single cell and then FACS-sorted to collect envelope and basal epithelial cells. Total RNA was extracted and ultra-low input RNA-seq with polyA enrichment was performed at the Medical Genomics Core at Indiana University.
Reads were mapped to GRCz11 using STAR (Dobin et al., 2013) with default parameters. BAM files generated were then used for transcript quantification with RSEM (Li and Dewey, 2011) using Lawson Lab Transcriptome annotation file, V4.3.2 (Lawson et al., 2020). Expression values showed in datasheet are average FPKM counts of three biological repeats, normalized on a log10 scale.
Transcripts that were undetected were assigned an arbitrary value of -4.00. Data can be searched by symbol or gene name and can be sorted by any column.
| Gene Names | Suprabasal layers of epithelial cells | Basal layers of epithelial cells |
|---|---|---|
| Gene Names | Suprabasal layers of epithelial cells | Basal layers of epithelial cells |