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portfolio
Fly Fishing: A Journey into the Art of the Cast
Explore the world of fly fishing, from its rich history to the thrill of catching steelhead trout and salmon.
Portfolio item number 2
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publications
A comprehensive single cell data analysis of lymphoblastoid cells reveals the role of super‐enhancers in maintaining EBV latency
Published in Journal of Medical Virology, 2022
This study delves into the lifecycle of Epstein-Barr virus (EBV) using single-cell RNA sequencing data from nine lymphoblastoid cell lines (LCLs). While most cells were in the latent phase, two distinct clusters emerged, characterized by the high expression of EBV genes LMP1/BNLF2 and BZLF1.
Recommended citation: Yan B, Wang C, Chakravorty S, et al. A comprehensive single cell data analysis of lymphoblastoid cells reveals the role of super-enhancers in maintaining EBV latency. J Med Virol. 2022;95:e28362. https://doi.org/10.1002/jmv.28362
Inwardly rectifying potassium channels regulate direction sensing during neutrophil chemotaxis
Published in Journal of Cell Biology (JCB), 2025
We demonstrate that the inwardly rectifying potassium channel Kir7.1 is essential for directional sensing during neutrophil chemotaxis, influencing membrane potential dynamics and GPCR signaling.
Recommended citation: Dr. Tianqi Wang, Mr. Daniel H Kim, Ms. Chang Ding, Mr. Dingxun Wang, Dr. Weiwei Zhang, Dr. Martin Silic, Dr. Xi Cheng, Mr. Kunming Shao, Mr. TingHsuan Ku, Mr. Conwy Zheng, Dr. Junkai Xie, Dr. Chongli Yuan, Dr. Alexander A Chubykin, Prof. Christopher J Staiger, Dr. Guangjun Zhang. Inwardly rectifying potassium channels regulate direction sensing during neutrophil chemotaxis. Journal of Cell Biology 2025. https://doi.org/10.1083/jcb.202503037
scChat: A large language model‐powered co‐pilot for contextualized single‐cell RNA sequencing analysis
Published in American Institute of Chemical Engineers (AIChE), 2026
We introduce scChat, a pioneering AI assistant designed for contextualized scRNA-seq analysis. Unlike existing tools that focus on standard tasks like cell annotation, scChat offers advanced capabilities, including research context-based analysis of experimental outcomes and suggestions for next-step experimental design.
Recommended citation: Chiu H-H, Varghese A, Shao K, et al. scChat: A large language model-powered co-pilot for contextualized single-cell RNA sequencing analysis. AIChE J. 2026;e70285. https://doi.org/10.1002/aic.70285
Photo-click Decellularized Matrix Hydrogels for Generating Pancreatic Ductal Organoids
Published in bioRxiv, 2026
We demonstrate that photo-clickable dSIS–norbornene (dSIS-NB) hydrogels can replace Matrigel to generate human iPSC-derived pancreatic ductal organoids, and that matrix stiffness is a key determinant of ductal fate.
Recommended citation: Hgoc Ha Luong, Kunming Shao, Van Thuy Duong, Xiaoping Bao, Chien-Chi Lin, bioRxiv 2026.02.16.706185 https://www.biorxiv.org/content/10.64898/2026.02.16.706185v1
CAR-neutrophils Produced In Vivo to Treat Glioma
Published in Nature Biomedical Engineering, 2026
We develop NeuSMRT, a neutrophil-specific modified RNA translation platform that enables in vivo generation of CAR-neutrophils and demonstrates therapeutic activity against glioma.
Recommended citation: Yun Chang, Kunming Shao, Huiyang Li, Gyuhyung Jin et al., Nature Biomedical Engineering, 2026 https://www.nature.com/articles/s41551-026-01656-0
Chemically Defined Production of CAR-neutrophils from Human Pluripotent Stem Cells
Published in Nature Protocols, 2026
We establish a chemically defined, feeder-free platform for engineering human pluripotent stem cells and differentiating them into functional CAR-neutrophils within 3 weeks.
Recommended citation: Kunming Shao, Zhaolun Liang, Jingqiao Shen, Gyuhyung Jin, Huiyang Li, Yan Tan, Conwy Zheng, Xiaojun Lian, Qing Deng, Xiaoping Bao, Nature Protocols
ANTS: an end-to-end automated platform for neutrophil chemotaxis imaging, tracking and behavioral profiling
Published in bioRxiv, 2026
We develop ANTS, an end-to-end platform integrating a 3D-printed chemotaxis device with deep-learning-based neutrophil tracking and automated behavioral profiling from label-free time-lapse imaging.
Recommended citation: Kunming Shao, Mengbo Wang, Conwy Zheng, Shaiv Mehra, Shourya Verma, Zhaolun Liang, Ding Chag, Xiaoping Bao, Ananth Grama, Qing Deng, bioRxiv
talks
Talk 1 on Relevant Topic in Your Field
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Conference Proceeding talk 3 on Relevant Topic in Your Field
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teaching
BIOL537 - Immunobiology
Graduate course, Purdue University, Biological Sciences, 2024
Course Description: Immunobiology
Zebrafish Bulk RNA-seq Analysis Pipeline
Teaching Resource, , 2024