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EDUCATION

B.S. Purdue University, West Lafayette, IN 2017 - 2021
Major: Biological Engineering / Cellular and Biomolecular Engineering
Minor: Biotechnology

Ph.D Candidate Purdue University, West Lafayette, IN 2022 - Present
Purdue University Interdisciplinary Life Science Program (PULSe)
Department of Biological Sciences
Major - Computational and Systems Biology

RESEARCH EXPERIENCE

Graduate Research Assistant.
Research Associate, Advisor: Dr. Qing Deng, Department of Biological Science
April 2023 – Present
o Elucidated microRNA-mediated regulatory networks governing the development and effector functions of iPSC-derived neutrophils
o Refined and scale up the iPSC-to-neutrophil differentiation pipeline to enhance cell yield, purity, and functional maturity

Research Associate, Co-Advisor: Dr. Xiaoping Bao, Department of Chemcial Engineering
April 2023 – Present
o Engineered neutrophil-like cells via nanoparticle-mediated delivery of modRNA
o Integrated machine learning pipelines to guide the rational design and optimization of CAR-engineered iPSC-neutrophils

Undergraduate Research Assistant.
Research Associate, Advisor: Dr. Majid Kazemian, Department of Biochemistry
August 2019 – Dec 2021
o Working on the novel noncoding RNA in viral associated cancers

Undergraduate Research Assistant.
Research Associate, Advisor: Dr. Luo Zhaoqing, Department of Biological Sciences
Dec 2018 – Dec 2019
o Study the mechanisms of how L. pneumophila modulates apoptotic pathways to promote its intracellular growth.

HONORS AND AWARDS

SKILLS

• Programming: Python, R, MATLAB, SQL, Linux/Unix Shell Scripting (Bash), LaTeX, ImageJ Macro, VBA, Git
• Software: FlowJo, FIJI/ImageJ, IGV, JMP, Jupyter Notebook, Google Colab, GraphPad Prism, Seurat, Scanpy, Cell Ranger, DESeq2, QuPath, Cellpose, DNA Master
• Molecular and Cellular Techniques: PCR, qPCR, RT-qPCR, DNA/RNA extraction, molecular cloning, immunofluorescence (IF), immunohistochemistry (IHC), confocal microscopy, ELISA, gel electrophoresis, mammalian cell culture, CRISPR/Cas9-mediated mammalian cell gene knock-in and out, Nucleic Acid Engineering (modRNA/gRNA design, IVT synthesis, and structural/integrity QC), western blotting(WB), flow cytometry, FACS sorting, nanoparticle formulation and characterization, extracellular vesicle (EV)/exosome isolation and characterization
• Stem Cells: hPSC/iPSC differentiation & Organoid engineering (Establishment, 3D culture, and maintenance), CRISPR/Cas9-mediated gene knock-in at the AAVS1 safe harbor locus; Viral vector engineering (AAV & Lentivirus design, packaging, and transduction).
• Omics: bulk RNA-seq, ATAC-seq, ChIP-seq, single-cell RNA-seq (scRNA-seq) , library preparation, data processing and analysis
• In vivo Animal Model: Mus musculus; Danio rerio
• Artificial Intelligence & Deep Learning: TensorFlow, PyTorch, LLM application engineering (API integration, RAG workflows, and local model deployment for biological data analysis)